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. Author manuscript; available in PMC: 2006 Jul 31.
Published in final edited form as: J Neurocytol. 2006 Jul 13;34(3-5):307–341. doi: 10.1007/s11068-005-8360-2

Fig. 10.

Fig. 10

Immunogold labeling for Cx43 in gap junctions of the pia mater and periglomerular ensheathing cells of adult mouse. (A) Angular gap junctions between layers of the pia mater, labeled for Cx43 (12 nm gold beads) but not for Cx45 (6 nm and 18 nm gold beads, none present). White arrows point to furrows corresponding to P-face images of tight junctions. Tight junctions typically surround and delineate gap junctions. (B) Low magnification image of a periglomerular ensheathing cell. Axon bundles (Ax) on the right side; nucleus (Nu) is at top left, and a Cx43-labeled gap junction is within the inscribed box. (C) Higher magnification view of the labeled gap junction in B. Connexons are arranged in small domains. (D) Low magnification view of periglomerular ensheathing cells. Nu = nucleus, PS = periglomerular space. Inscribed area “E” shown at higher magnification below. (E) Cx43-labeled gap junctions (12 mm gold beads) and “reciprocal patches” (black arrowhead) in E-face of the plasma membrane of a periglomerular ensheathing cell.