Figure 10. Effect of calcium channel blockers.

Cells were incubated in the absence (Control) or presence of Hcy (100 μM) for 30 min. In some experiments, cells were pre-incubated with verapamil (Ver; 10 nM) or diltiazem (Dil; 100 nM) for 30 min, followed by incubation with Hcy (100 μM) or without Hcy for another 30 min. (A) Serine-phosphorylated p47^phox and p67^phox proteins were determined by immunoprecipitation and immunoblotting analysis. Results were normalized to total p47^phox and p67^phox proteins in the immunoprecipitates. (B) Intracellular superoxide anion levels were measured by the NBT reduction assay. The level of superoxide anions in control cells was expressed as 100%. Results are expressed as means±S.E.M. for four separate experiments each performed in duplicate. *Significantly different from the control value (P<0.05). ^#Significantly different from the value obtained from Hcy-treated cells (P<0.05).