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. 2006 Jul 27;398(Pt 1):73–82. doi: 10.1042/BJ20051810

Figure 9. Effect of LY333531 on PKC activity, NADPH oxidase subunit phosphorylation and superoxide anion levels in monocytes.

Figure 9

(A) Cells were incubated in the absence (Control) or presence of Hcy (100 μM) for 30 min. In some experiments, cells were pre-incubated with a PKC inhibitor (LY333531; 5 nM) for 30 min, followed by incubation with Hcy (100 μM) or without Hcy for another 30 min. PKC activity was measured. (B) Serine-phosphorylated p47phox and p67phox protein subunits were determined by immunoprecipitation and Western-blot analysis. Results are expressed as means±S.E.M. for three separate experiments. (C) Intracellular superoxide anion levels were measured by the NBT reduction assay. The level of superoxide anions in control cells was expressed as 100%. Results are expressed as means±S.E.M. for four separate experiments each performed in duplicate. The values obtained from control cells were expressed as an arbitrary unit of 100%. *Significantly different from the control value (P<0.05). #Significantly different from the value obtained from Hcy-treated cells (P<0.05).