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Spectrophotometric analysis in purified PDHc from porcine heart demonstrates uncompetitive inhibition of PDHc activity by acetyl-CoA with respect to pyruvate (Pyr) as demonstrated by a Lineweaver–Burk plot. Values are given as the means±S.D. (where no error bars are visible the S.D. is within the symbol). Experiments were performed at least in triplicate.
