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. 2006 Jul 27;398(Pt 1):125–133. doi: 10.1042/BJ20060109

Figure 1. Purification of CAR complex from nuclear extracts of Ym17 cells.

Figure 1

Protein complexes were purified using a V5 antibody (α-V5) column or normal mouse IgG (nmIgG) column. The nuclear extract (NE), flow-through (FT) fraction and the fraction eluted with 0.2 M glycine/HCl (pH 2.8) containing 0.1% Triton X-100 (E) were applied to an SDS/4–12% polyacrylamide gel. (A) Western-blot analysis was performed using the V5 antibody to detect mCAR. (B) The protein band of mCAR was visualized by silver staining. (C) The band corresponding to SMC1 was stained by CBB.