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. 2006 Jul 27;398(Pt 1):113–124. doi: 10.1042/BJ20060280

Figure 3. Cytokinin intermediate formation and decay.

Figure 3

Time courses of the reaction intermediate formation and decay (310 nm; circles) and oxidation changes of the FAD cofactor (451 nm; triangles) observed for ZmCKX1 (5 μM) that was mixed aerobically with 125 μM cytokinins [(a) isopentenyladenine, (b) N-methyl-isopentenyladenine, and (c) trans-zeatin] in 75 mM imidazole/HCl (pH 6.5; left-hand panels) and 75 mM Tris/HCl (pH 8.0; right-hand panels). The data could be fitted using a model in which species A is converted in a hyperbolic fashion into species B that then decays exponentially to form C.