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. 2006 Jul 27;398(Pt 1):83–95. doi: 10.1042/BJ20060509

Figure 3. MEK dominant-negative mutation affects both VV- and CPV-stimulated ERK1/2 phosphorylation and EGR-1 expression.

Figure 3

Upper panels: cell lines expressing WT MEK or MEK dominant-negative mutation (DN) were either stimulated with EGF (50 μg/ml) for 30 min or infected with VV or CPV at an MOI of 10.0 for 4 h (A) or at an MOI of 10 for the indicated times (B and C). Whole-cell lysates were prepared and immunoblotted with anti-phospho-ERK1/2 (A) or anti-EGR-1 (B and C) antibodies. Lower panels: blots were re-probed with anti-(total ERK1/2) antibody as an internal control for protein loading. Blots are representative of at least three independent experiments with similar results.