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. 2006 Jul 27;398(Pt 1):83–95. doi: 10.1042/BJ20060509

Figure 5. Upon nuclear translocation, EGR-1 binds to the regulatory sequence EBS.

Figure 5

EMSA carried out with labelled EBS probe. Whole-cell extract (10 μg per sample) was mixed with the probe. Lanes 1–4 and 13, mock-infected; lane 5, stimulated with 10% FBS for 30 min; lanes 6–12 and 14–17, VV-infected for the times shown; lanes 11, 12 and 15, incubated with PD98059 (50 μM) before virus infection; lanes 16 and 17, competed with 50-fold molar excess of unlabelled EBS or NF-κB oligonucleotides respectively; lane 18, incubated with anti-EGR-1 antibody for 15 min before virus infection. Data were confirmed experimentally three times with similar results.