Table 2. Kinetic parameters for the synthesis reactions of CgCBP with different glucosyl acceptors, and interactions in the complex structure.

The ΔΔG is the Gibbs energy of activation for k_cat/K_m for the enzymatic glucosyl transfer to an acceptor, relative to transfer to D-glucose, was calculated according to ΔΔG=RTln[(k_cat/K_m)_D-glucose/(k_cat/K_m)_acceptor], whereby R is 1.987 cal·mol⁻¹·K⁻¹ and T is 310.15 K [39]. Values in parentheses are taken from the results of CuCBP (T is 303.15 K) [32]. Values for 1,5-anhydroglucitol, isomaltose, gentiobiose and melibiose were determined in the present study.

		Km (mM)	kcat (s−1)	kcat/Km (s−1·M−1)	ΔΔG (kcal/mol)		Hydrogen-bond pair	Distance (Å)
	Glucose [11]	2.1	98	46800
O-1	1,5-Anhydroglucitol	89	5.3	59	4.11	(>7)	Glu649 Oϵ	2.6
O-2	Mannose [11]	115	39	342	3.03	(2.65)	Tyr653 Oη	2.8
	2-Deoxyglucose [11]	168	37	220	3.30	(2.24)	Glu659 Oϵ1	3.0
	Glucosamine [11]	13	15	1160	2.28	(2.19)
O-3	(3-Deoxyglucose)					(4.01)	Lys658 Nζ	3.0
							Glu659 Oϵ2	2.7
O-4							Asp490 Oδ	2.6
O-5	5a-Carba-β-D-glucopyranose [12]	55	93	1680	2.05		Gln165
							(other monomer) Nϵ	3.2
O-6	6-Deoxyglucose [11]	24	139	5780	1.29	(0.77)	None
	Xylose [11]	84	31	372	2.98	(2.15)
	Isomaltose	71	1.9	27
	Gentiobiose	83	7.2	87
	Melibiose	93	2.3	24