Figure 4. The effect of cell matrix and Rho-dependent signalling on nuclear LPA₁ in PC12 cells.

Serum-deprived PC12 cells were immunostained using anti-LPA₁^328–344 antibody and detected with anti-(rabbit FITC-conjugated) secondary antibody (green). Actin was stained using phallodin red (red). (A) Serum-deprived PC12 cells were treated with 25 μM RGDS or 25 μM RGES for 24 h; or were grown on (B) 10 μg/ml fibronectin (FN) or 0.01% polylysine (PLL)-coated plates; or (C) were treated with 10 μM Y27632 or 10 μM ML9 for 4 h or untreated. Results shown are a single representative of three experiments.