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Freshly prepared nuclei in low-speed nuclear pellets from serum-deprived PC12 cells were untreated or treated with 10 μM Ki16425 for 20 min, or with 5 μM LPA for 5 min, or with 10 μM Ki16425 for 20 min followed by 5μM LPA for 5 min. The Western blots show the effect of Ki16425 and/or LPA on (A) nuclear protein serine/threonine phosphorylation and (B) nuclear protein tyrosine phosphorylation.
