Table 2.
Beat frequency of cpc1 flagella in vivo and in vitro
| Beat frequency ± s.d. (n), (Hz)
|
||||
|---|---|---|---|---|
| Sample | Nucleotide added | Wild type | cpc1 | % cpc1/wild type |
| Live cells | – | 58±5 (45) | 37±5 (45)a | 64% |
| Cell model | 1 mM ATP | 43±7 (40) | 38±5 (40)a | 88% |
| Cell model | 1 mM ADP | 20±2 (20) | 19±3 (20) | (100%)b |
| Axonemes (Exp. 1) | 1 mM ATP | 58±3 (40) | 50±5 (40)a | 86% |
| Axonemes (Exp. 2) | 1 mM ATP | 52±5 (96) | 49±4 (96)a | 94% |
| Axonemes | 1 mM ADP | 22±3 (40) | 23±3 (36) | (100%)b |
a
Significantly different from the wild-type beat frequency (P<0.01, two-tailed t test).
b
No significant difference between mutant and wild-type beat frequency.