Fig. 1.

Effects of dsRNA-mediated gene knockdown on the dual-luciferase-based RNAi reporter assay. RL activity was normalized to that of Firefly (y axis) in response to dsRNA-targeting Renilla (dsRL). (A) Effects on RNAi response (and its time dependence) after knockdown of genes implicated in the miRNA or siRNA pathway. Normalized Renilla/FL activities are compared with the ratio of these activities in the absence of dsRL (YFP columns). Reporter constructs were transfected 48 h (black columns) or 96 h (gray columns) after the initial bathing with dsRNA targeting the gene of interest (indicated on x axis). (B) Luminescence readings (Renilla/Firefly) from the 96-well plate where AGO2 is targeted by dsRNA (gray column, position E07). Column 1 represents the control where no dsRL has been added.