Fig. 2.

Effect of dsRNA-mediated gene knockdown of seven candidate genes (and YFP control) on dsRNA-mediated silencing of the endogenous gene SREBP. S2 cells were treated first with dsRNA targeting the candidate genes (as indicated) and then, after 48 h, with dsRNA targeting SREBP (except for lane 1). (A) Western blot analysis of protein isolated from treated cells probed with anti-SREBP and anti-Tubulin antibodies. (B) Northern blot analysis of total RNA isolated from treated cells probed for SREBP and rp49 mRNAs. The protein and RNA analyses were performed twice in duplicate.