Table 1.

Fold increase of normalized RL in knockdown cells

Z value	Gene targeted	First RNA	Second RNA	Third RNA	Predicted/known function
9.5	CG7439	78 ± 6	24 ± 7		Ago2, RNAi pathway
9.5	Tis11	19 ± 3	15 ± 3	2.1 ± 0.6	RNA catabolism, RNA binding
9.5	CG7239	14 ± 4	1.4 ± 0.5
9.3	Hsc70-4	9 ± 2	30 ± 10		Heat shock protein cognate
9.1	CG15074	13 ± 3	0.9 ± 0.4		Heparin-glucosamine 3-O-sulfotransferase activity
9.1	ab	8 ± 1	0.8 ± 0.2		Transcription factor activity
9.1	CG14358	7 ± 3	0.8 ± 0.1
9.0	fru	19 ± 2	1.4 ± 0.7		Transcription factor activity
8.6	CG12969	25 ± 2	1.0 ± 0.1		Actin binding
8.4	hdc	32 ± 2	25 ± 4		Trachea development
8.3	CG6460	7 ± 2	0.8 ± 0.2
8.2	CG17265	59 ± 6	14 ± 3	2.0 ± 0.2
8.1	Lim3	12 ± 4	1.5 ± 0.4		Transcription factor activity
7.6	Eip75B	9 ± 4	0.5 ± 0.1		Transcription factor activity
7.5	m2	14 ± 1	1.1 ± 0.1		Notch signaling pathway
7.1	CG7173	9 ± 4	1.1 ± 0.4
6.8	CG2556	24 ± 5	1.1 ± 0.4
6.4	Hsc70-3	25 ± 6	35 ± 6		Heat shock protein cognate
6.4	CG5060	12 ± 3	1.2 ± 0.6
6.3	CG8154	9 ± 3	0.8 ± 0.4
6.1	CG13188	12 ± 2	1.3 ± 0.2
5.6	CG10883	2.4 ± 0.6	2.1 ± 0.3
5.6	CG5732	6 ± 2	0.9 ± 0.5		Polynucleotide adenylyltransferase activity
4.1	EG:132E8.1	3 ± 1	1.6 ± 0.3		mRNA binding
4.6	HLHm3	6 ± 1	0.9 ± 0.2		Notch signaling pathway

Z values were calculated from the primary screen. Values represent the average of a triplicate experiment. Experiments were performed multiple times. Luminometer values were normalized (Renilla/Firefly activity) and compared with the ratio of activities of cells treated with the dsYFP control RNA in the bathing step. Predicted/known functions are listed as found in Flybase (46). Seven of the original candidate genes (bold) were confirmed based on their reproducible effects.