FIG. 3.
Artificially created lysis reporter strain of Acinetobacter strain ADP1 responding to hexadecane, an inducer of the alkM gene promoter. (A) Structure of the lysis cassette of bacteriophage λ, devoid of promoter and ligated to a Kmr gene. This construct was inserted into the chromosome of Acinetobacter strain ADP1 under the control of the alkM promoter. (B) Release of DNA from the obtained strain. Cells were grown to an OD600 0.4, and hexadecane was added at the indicated concentrations. After overnight incubation, cells were removed by centrifugation, and DNA released into the medium was isolated as described in Materials and Methods and separated by agarose gel electrophoresis. M, DNA molecular mass markers, with the upper band corresponding to a 10-kB DNA fragment.