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Localization of aadD2 in B. clausii. Total DNA from B. clausii strains NR (lanes 1), OC (lanes 2), T (lanes 3), SIN (lanes 4), and DSM8716 (lanes 5) was digested with restriction enzyme I-CeuI and subjected to pulsed-field gel electrophoresis. DNA was transferred to a nylon membrane and hybridized successively with rrs (16S rRNA) (left) and aadD2 (right) probes labeled with digoxigenin. The low-molecular-weight I-CeuI band is barely visible in lanes 2, 3, 4, and 5 (left).
