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. 2006 Jul 14;7(8):824–830. doi: 10.1038/sj.embor.7400751

Figure 2.

Figure 2

Efficient Otx2 deletion after single tamoxifen injection. (A) Morphology of animals of indicated age and genotype after tamoxifen (Tam) or solvent (Mock) injection at embryonic day (E)7.5. Scale bars, 500 μm. (B) PCR detection of flox and deleted exon 2 (Δe2) alleles (for primers, see supplementary Fig S1 online) using DNA from the brain or head of embryos of indicated age and genotype. (C) Alkaline phosphatase activity in E9.5 Otx2+/CreERT2 ; Z/AP embryos that received tamoxifen or solvent at E7.5. Scale bar, 500 μm. (D) PCR analysis, as in (B), using DNA from the indicated part, and age of Otx2flox/CreERT2 embryos after tamoxifen (Tam) or solvent (mock) injection at the indicated stages. (E–L) In situ hybridization with Otx2 exon 2 probe and CreERT2 probe (insets; for probes, see supplementary Figs S1,S2 online) of sagittal sections of Otx2flox/CreERT2 embryos that received tamoxifen (Tam) or solvent (Mock) at indicated times and were collected 30 h later. Scale bars, 1 mm.