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piggyBac vectors containing the β-globin 5' HS4 and gypsy insulator elements. The vectors contain either the ECFP (A), EYFP (B) or DsRed1 (C) marker genes. The β-globin 5' HS4 (A, B) and gypsy (C) insulator elements do not bracket the marker genes but do flank the unique AscI and FseI cloning sites. The vectors have 685 bp of 5' terminal piggyBac sequence and 1051 bp of 3' terminal sequence.
