Table 2.
Fluorescence intensities of soluble protein extracts prepared from heads of transgenic lines expressing reef coral fluorescent proteins.
| Line | Markera | Insulatedb | Mean Fluorescence/μg proteinc | SD |
| A1 | GMR-3xP3-AmCyan | Yes | 857 | 173 |
| A2 | GMR-3xP3-AmCyan | Yes | 629 | 69 |
| A3 | GMR-3xP3-AmCyan | Yes | 846 | 88 |
| A4 | GMR-3xP3-AmCyan | Yes | 1065 | 257 |
| A5 | GMR-3xP3-AmCyan | Yes | 1016 | 83 |
| y w d | None | 13 | 37 | |
| A7 | GMR-3xP3-ZsGreen | Yes | 758 | 71 |
| A8M1 | GMR-3xP3-ZsGreen | Yes | 828 | 41 |
| A8M3 | GMR-3xP3-ZsGreen | Yes | 1558 | 20 |
| A9 | GMR-3xP3-ZsGreen | Yes | 1205 | 176 |
| A10 | GMR-3xP3-ZsGreen | Yes | 1213 | 185 |
| A11 | GMR-3xP3-ZsGreen | Yes | 1737 | 82 |
| y w | None | 41 | 43 | |
| R31 | 3xP3-DsRed1 | No | 118 | 24 |
| R30A | 3xP3-DsRed1 | No | 859 | 56 |
| R19 | 3xP3-DsRed1 | No | 215 | 135 |
| R18 | 3xP3-DsRed1 | No | 169 | 51 |
| R12 | 3xP3-DsRed1 | No | 171 | 31 |
| R21 | 3xP3-DsRed1 | No | 342 | 14 |
| y w | None | 122 | 43 |
aAll lines heterozygous for marker gene
bscs and scs' insulators bracket the GMR-3xP3 marker genes.
cAverage of three protein extracts each prepared from ten homogenized heads from each line. Fluorescence in arbitrary units.
dParental stock used in all germ-line transformation experiments