Table 2.
Reverse transcriptase-PCR analysis of the expression of clusters and contigs differentially expressed between the L1 and iL3s stages.
| Cluster 1 | Fold 1 | Contig 1 | Fold 1 | RT-PCR mean (SD) 2 |
| SR00369 | 3.6 | SR01101 | 3.89 | 0.84 (0) |
| SR00073 | 2.46 | SR01013 | 2.46 | 1.02 (0) |
| SR00647 | 3.68 | SR00647 | 3.68 | 3.13 (3.7) |
| SR00369 | 3.6 | SR01099 | 3.1 | 4.34 (4.2) |
| SR00001 | 2.83 | SR00001 | 5.82 | 4.18 (0.2) |
| SR00369 | 3.6 | SR01100 | 5.82 | 1.7 (1.0) |
| SR00762 | 3.2 | SR00762 | 3.2 | 18.7 (0.14) |
| SR00369 | 3.6 | SR00853 | 4.24 | 5.74 (6.6) |
| SR00222 | 4.46 | SR00222 | 4.45 | 18.3 (5.8) |
| SR03050 | 3.51 | SR03050 | 3.51 | 2.69 (2.3) |
| SR01720 | 2.93 | SR01720 | 2.93 | 9.7 (3.9) |
| SR00448 | 2.60 | SR00448 | 2.59 | 9.6 (12.5) |
| SR01335 | 2.22 | SR01335 | 2.22 | 3.63 (4.0) |
| SR00906 | 2.06 | SR00906 | 2.06 | 3.39 (2.8) |
| SR02481 | 1.85 | SR02481 | 1.85 | 7.61 (0.71) |
| SR01791 | 1.64 | SR01791 | 1.64 | 2.4 (1.3) |
| SR00681 | 1.52 | SR00681 | 1.52 | 4.3 (3.7) |
| SR01787 | 1.42 | SR01787 | 1.42 | 2.72 (3.7) |
| SR00322 | 1.16 | SR00322 | 1.16 | 8.08 (9.6) |
| SR00026 | 2.06 | SR00172 | 2.88 | 6.71 (0) |
| SR00026 | 2.06 | SR00047 | 2.33 | 1.77 (0.22) |
| SR00012 | 2.27 | SR01022 | 2.74 | 0.12 (0) |
| SR01012 | 2.5 | SR01012 | 2.5 | 6.5 (0) |
| SR00946 | 1.96 | SR01050 | 2.02 | 8.75 (10.3) |
| SR00012 | 2.27 | SR00685 | 3.1 | 15.9 (20.1) |
| SR01053 | 1.32 | SR01053 | 1.32 | 2.44 (2.9) |
| SR00880 | 3.13 | SR00880 | 3.13 | 5.63 (6.9) |
| SR00978 | 2.2 | SR00978 | 2.2 | 2.86 (2.8) |
| SR01196 | 3.65 | SR01196 | 3.66 | 10 (8.5) |
| SR02281 | 2 | SR02281 | 2 | 3.22 (3.2) |
| SR00930 | 1.87 | SR00930 | 1.87 | 1.04 (0.31) |
| SR01051 | 1.71 | SR01051 | 1.71 | 1.18 (0.9) |
| SR00957 | 1.44 | SR00957 | 1.44 | 3.41 (1.9) |
| SR02851 | 1.43 | SR02851 | 1.43 | 1.45 (0.32) |
| SR00194 | 1.42 | SR00194 | 1.42 | 0.28 (0.39) |
| SR00084 | 1.39 | SR01021 | 1.39 | 6.34 (5.5) |
| SR00795 | 1.2 | SR00795 | 1.2 | 3.97 (0) |
| SR00230 | 1.11 | SR00230 | 1.11 | 5.39 (2.7) |
| SR00163* | 1.91 | SR00785 | 3.12 | 0.89 (0.12) |
| SR00113* | 1.54 | SR00113 | - | 0.99 (0.15) |
1Mean cluster and contig fold difference in expression between L1 and iL3 stages measured by microarrays for iL3-up (normal text) and L1-up (bold text) clusters and contigs and for reference genes (*); – indicates that there were no significant data generated for this contig.
2The mean (SD) fold difference in expression as measured by RT-PCR is calculated as iL3s/L1 for iL3-up (normal text) contigs and clusters and L1/iL3 for L1-up (bold text) clusters and contigs and for the reference genes (*).