Table 7.
Reverse transcriptase-PCR analysis of the expression of clusters and contigs differentially expressed between the directly and indirectly developing L2s.
| Cluster 1 | Fold 1 | Contig 1 | Fold 1 | RT-PCR Mean (SD) 2 |
| SR02363 | 2.08 | SR02363 | 2.08 | 1.45 (0.46) |
| SR00009 | 1.69 | SR00027 | 1.69 | 0.76 (0.45) |
| SR00113 | 1.60 | SR00113 | 1.58 | 1.30 (0.67) |
| SR00113 | 1.60 | SR00987 | 1.63 | 1.21 (0.18) |
| SR00013 | 1.25 | SR00053 | 1.26 | 0.95 (0.48) |
| SR02856 | 1.48 | SR02856 | 1.48 | 2.69 (2.06) |
| SR00395 | 1.46 | SR00395 | 1.46 | 1.81 (2) |
| SR00012 | 1.40 | SR00989 | 1.45 | 0.66 (0.17) |
| SR00012 | 1.40 | SR00665 | 1.39 | 0.99 (0.59) |
| SR01030 | 1.39 | SR01030 | 1.39 | 1.09 (0.6) |
| SR00008 | 1.31 | SR00752 | 1.31 | 1.81 (1.01) |
| SR01870 | 1.34 | SR01870 | 1.34 | 1.59 (2.06) |
| SR00591 | 1.30 | SR00591 | 1.30 | 0.75 (0.33) |
| SR00012 | 1.40 | SR00955 | 1.28 | 1.30 (0.35) |
| SR00055 | 1.23 | SR00962 | 1.23 | 0.97 (0.14) |
| SR00112 | 1.21 | SR00112 | 1.21 | 3.86 (5.72) |
| SR00950 | 1.18 | SR00950 | 1.18 | 1.46 (1.45) |
| SR01054 | 1.18 | SR01054 | 1.18 | 2.98 (3.91) |
| SR00611 | 1.16 | SR00611 | 1.16 | 4.38 (2.89) |
| SR00038 | 1.22 | SR00038 | 1.13 | 0.97 (0.51) |
| SR00205 | 1.01 | SR00205 | 1.01 | 0.38 (0.33) |
| SR00170 | 2.58 | SR00170 | 2.58 | 6.18 (1.76) |
| SR00374 | 2.02 | SR00374 | 2.02 | 7.27 (4.57) |
| SR00351 | 1.73 | SR00931 | 1.83 | 12.9 (20.3) |
| SR00713 | 1.74 | SR00713 | 1.74 | 1.13 (0.36) |
| SR00775 | 1.68 | SR00775 | 1.68 | 6.28 (8.64) |
| SR00199 | 1.45 | SR00199 | 1.45 | 1.71 (0.22) |
| SR01250 | 1.38 | SR01250 | 1.38 | 3.51 (0) |
| SR00799 | 1.35 | SR00799 | 1.35 | 4.38 (4.28) |
| SR00811 | 1.28 | SR00811 | 1.28 | 0.91 (0.3) |
| SR00092 | 1.21 | SR00092 | 1.21 | 0.99 (0.24) |
| SR02792 | 1.21 | SR02792 | 1.21 | 6.56 (7.21) |
| SR00721 | 1.19 | SR00721 | 1.19 | 1.06 (0.27) |
| SR02972 | 1.17 | SR02972 | 1.17 | 14.8 (23.1) |
| SR00994 | 1.16 | SR00994 | 1.16 | 1.16 (0.43) |
| SR01156 | 1.57 | SR01156 | 1.57 | 2.04 (2.06) |
| SR02019 | 1.84 | SR02019 | 1.84 | 2.85 (3.94) |
| SR00178 | 1.39 | SR00178 | 1.39 | 1.09 (0.11) |
| SR00342 | 1.14 | SR00543 | 1.14 | 10.8 (1.5) |
| SR02917 | 1.1 | SR02917 | 1.1 | 26.5 (41.6) |
| SR00615 | 1.15 | SR00615 | 1.1 | 1.76 (0.65) |
| SR02394 | 1.07 | SR02394 | 1.07 | 8.72 (1.75) |
| SR00817 | 1.06 | SR00817 | 1.06 | 13.4 (1.11) |
| SR00266 | 1.06 | SR00266 | 1.06 | 2.55 (2.5) |
| SR00451 | 1.02 | SR00451 | 1.02 | 11.1 (16.2) |
| SR00113* | 1.6 | SR00113 | 1.58 | 1.23 (0.87) |
| SR00015* | 1.1 | SR00015 | 1.1 | 0.25 (0.01) |
1Mean cluster and contig fold difference in expression between indirectly and directly developing L2 stages measured by microarrays for L2 direct-up (normal text) and L2 indirect-up (bold text) clusters and contigs and for reference genes (*).
2 The mean (SD) fold difference in expression as measured by RT-PCR is calculated as direct/indirect for the L2 direct-up (normal text) contigs and clusters and for the reference genes (*) and indirect/direct for the L2 indirect-up (bold text) contigs and clusters.