FIG. 1.

Kinetics of gef and chpBK induction. Cells of E. coli ERAPlacI harboring (A) pUPRIP, (B) pGef, and (C) pChpBK were grown overnight at 37°C and then diluted 1:100 into fresh LB medium containing appropriate antibiotics. At early log phase (OD₆₀₀ of 0.3 to 0.4), the culture was divided equally (indicated by arrow) and incubated in the presence (•) or absence (○) of 1 mM IPTG. The growth of the culture was monitored spectrophotometrically at a wavelength of 600 nm. Each graph is representative of at least two experiments.