Figure 5.
Live cell analysis of EB1-GFP emergence from the centrosome. (A) EB1-GFP was detected as bright fluorescent spots (long arrow) emanating from the centrosome (short arrow) over time (seconds). Bar, 5 μm. (B) MT nucleation rate was measured by the frequency of EB1-GFP comet emergence from the centrosome over time (minute−1). Cells infected for 15–45 min with Ad (dark bars) exhibited a significantly higher rate of MT nucleation compared with uninfected cells (white bars) in both A549 cells (p = 3 × 10−11) and HFF cells (p = 1 × 10−7). Each bar represents the mean frequency from 20 to 40 distinct measurements per condition ±SEM.