Figure 2.

Hsp90 activity is required for extraction of Rab1 in vitro by αGDI. Extraction of Rab1 by increasing concentrations of recombinant αGDI from semi-intact NRK cells was performed as described previously (Peter et al., 1994). To test the effect of Hsp90 inhibitors, cells were pretreated with 20 μM GA or 50 μM radicicol (Rad) for 60 min before preparation of semi-intact cells. A typical experiment is shown in the inset. Quantitation based on the average of three independent experiments. Error bars indicate SEM.