Figure 5.

Overexpression of Rab1 protein antagonizes the inhibitory effect of radicicol on VSV-G^ts transport. (A) CHO-K1 cells were transfected with pcDNA3.1 plasmids containing Rab1B, Rab3A, Sar1A, or empty vector 24 h before infection with VSV^ts as described in Materials and Methods. Infected cells were then pulse-labeled in the absence or presence of radicicol (Rad). Representative autoradiograph is shown. (B) Quantitation based on two independent experiments with triplicate samples. Reported as percentage of increase in endo H-resistant VSV-G^ts normalized to mock control to illustrate fold-difference in recovery. (C) Immunoblot analysis of transfected cell lysates demonstrating expression of each of the GTPases relative to endogenous levels. Transient expression did not significantly affect the endogenous level of Hsp90, GDI or alter the total amount of VSV-G^ts synthesized.