FIG. 3.
Diagrams of expression constructs used in this work. The genes have been obtained from S. nogalater (sno; black), S. galilaeus (akn; gray), and Streptomyces PGA64 (pga; white); and they encode the following proteins: KSα (snoa1, aknB), KSβ (snoa2, aknC), ACP (snoa3, aknD), starter unit selection (aknE2, aknF), KR (snoaD), ARO (snoaE, pgaL), and CYC (pgaF). The antibiotic biosynthesis genes were cloned in the pIJ486 vector under the constitutive ermE promoter (8).