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Kinetics of [3H]AMP phosphorylation by lymphoid cells. (A) Time course of conversion of 50 μM [3H]AMP into [3H]ADP/ATP by Jurkat cells (mean ± SEM; n = 4). (B) The extent of [3H]AMP phosphorylation versus substrate concentration plots. Values are the mean ± SEM for Jurkat (Jurk; n = 8), lck-deficient Jurkat mutants J.CaM1.6 (JCaM; n = 3), Namalwa (Nam; n = 3), and freshly isolated human PBL (n = 3).
