Figure 4.

Effect of various agents on intrinsic [³H]AMP-phosphorylating capacity and adenylate kinase activity in lymphocytes. Jurkat (A) and Namalwa (B) were pretreated for 20 min with the indicated compounds followed by addition of either 50 μM [³H]AMP alone (■) or 400 μM [³H]AMP plus 800 μM ATP (▩) for measuring the intrinsic AMP-phosphorylating capacity and specific ecto-AK activity, respectively. The amount of [³H]AMP converted into [³H]ADP/ATP after a 45-min incubation with lymphocytes was determined by TLC and is represented as percentage of controls (mean ± SEM), with the number of experiments in parentheses. * p < 0.05 compared with nontreated control cells. ND, not determined.