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. 2006 Aug;17(8):3534–3542. doi: 10.1091/mbc.E05-11-1082

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© 2006 by The American Society for Cell Biology

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Figure 6. — Apoptosis assay of LRRC4 in the mock- and LRRC4-transfected U251cells. (A) Flow cytometry histogram displaying apoptosis apex in front of G₁ phase. (B) AO/EB dual staining showing necrosis cells and apoptosis cells. (C) Western blotting image showing caspase-8 and caspase-3 at the protein level in the mock- and LRRC4-transfected U251 cells (+/− serum). β-actin is used as the loading control. (D) Real-time PCR products displaying caspase-8 and caspase-3 mRNA in the mock- and LRRC4-transfected U251 cells.