Figure 1.

CpG-specific methylation level at the LEP, PPARG2, FABP4, and LPL promoters in cultured ASCs. (A) Distribution of CpGs in each promoter region examined. Numbers indicate nucleotide number upstream of the ATG translational start site (see Supplemental Figure S1 for sequences). Tick marks indicate the position of each CpG. (B–E) Percentage of 5′–3′ CpG methylation determined by bisulfite sequencing at indicated promoters in polyclonal populations of cultured undifferentiated ASCs. Number 1 refers to the 5′-most CpG.