Figure 1.

VEGF induces phosphorylation of FAK on serine residues. (A) Quiescent HUVECs were treated or not with 5 ng/ml VEGF for increasing periods. Proteins were extracted and were separated by SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was processed for immunodetection of phospho-FAK Ser722, Ser732, Ser843, Ser910, and total FAK. (B) Quiescent HUVECs were treated or not with 5 ng/ml VEGF for increasing periods. Proteins were extracted as in A and were processed for immunodetection of phospho-FAK Ser732, Tyr407, and total FAK. Representative blots are shown at the top and quantification of the blots (means ± SD) of three separate experiments is shown at the bottom. Arrows denote the bands that specifically correspond to phospho-Ser732 and phospho-Tyr407 (see Materials and Methods) and that were used for quantification.