Figure 6.

Tubular clusters stain for EEA1 and TF but not Rab 11. A431 cells were nucleofected with control oligo (Inv) or Tsg101 siRNA (Tsg101). Cells were stimulated with either fluorescent EGF for 30 min (A and B) and chased for 2 h or stimulated with fluorescent TF for 2 h (C). Cells were fixed and stained for EEA1 (A and C) or Rab11 (B). Tsg101 depletion causes clustering of EEA1 and TF into the same compartment as EGF. EEA1- and TF-positive clusters are reduced but still present in non-EGF–stimulated cells (C). Bars, 10 μm.