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Tsg101 depletion reduces MVB formation. A431 cells were nucleofected with control (Inv) or Tsg101 siRNA and incubated with anti-EGFR gold antibody and EGF for 0 min to 2 h. Cells were analyzed by EM (see Materials and Methods). There was a small reduction in MVB numbers in the absence of EGF stimulation, and EGF-stimulated MVB formation was abolished. Data shown represent mean ± SEM number of MVBs per square micrometer of the cytoplasm from three independent experiments. (∗p < 0.05).
