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Colocalization analysis of shrew-1-GFP with rab GTPases rab5 and rab11. (A–F) MCF-7 cells were transfected with shrew-1-GFP, fixed 24 h later, and stained with anti-rab5 or anti-rab11 antibodies. Bound anti-rab5 and anti-rab11 antibodies were detected using Alexa-Fluor 594 anti-rabbit antibody. Shrew-1-GFP colocalized with rab11 (C) and displayed no colocalization with rab5 (F).
