Figure 2.

Analysis of hnRNP A2-dependent recruitment of hnRNP E1 to A2RE RNA by cross-correlation FCS. Recombinant hnRNP E1, labeled with Alexa Fluor 647, was incubated in the presence (closed circles) or absence (open circles) of unlabeled hnRNP A2 (10 nM), with synthetic RNA containing 12 copies of the A2RE sequence (5 nM). Similarly labeled hnRNP E1 was incubated in the presence (closed triangles) or absence (open triangles) of unlabeled hnRNP A2 (10 nM), with control nonA2RE RNA (5 nM) labeled with Alexa Fluor 488. Unlabeled tRNA (250 nM) was included to reduce nonspecific binding. Recruitment of hnRNP E1 to RNA was determined by dual-channel cross-correlation FCS.