Figure 4.

Effect of hnRNP E1 overexpression on translation. (A) B104 cells injected with GFP-A2RE RNA. (B) B104 cells injected with hnRNP E1 cDNA and GFP-A2RE RNA. (C) B104 cells injected with GFP-A8G RNA; (D) B104 cells injected with hnRNP E1 cDNA and GFP-A8G RNA. All RNAs were polyadenylated, and all the injection mixtures contained Alexa Fluor 546-conjugated dextran. Newly synthesized GFP was visualized in the green channel, and Alexa Fluor 546-conjugated dextran in the red channel. The average intensity in each channel was determined and plotted for each cell. Each dot represents one cell. A representative experiment is shown in which 68, 63, 48, and 64 cells were analyzed in A, B, C, and D, respectively.