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. 2003 Apr;47(4):1430–1432. doi: 10.1128/AAC.47.4.1430-1432.2003

FIG. 2.

FIG. 2.

Comparison of the visible absorption UV-Vis spectrum. Spectral changes were observed upon adding NADPH (1 mM) to incubation mixtures containing tetracycline (30 μg/ml) and cell extract of E. coli containing tet(X) before incubation (-×-) and after 90 min of incubation (-○-) and containing tet(37) before incubation (—) and after 90 min of incubation (--▵--). E. coli cell extracts containing tetracycline and the vector only showed no change in absorbance after 90 min of incubation (—▪—). O.D., optical density.