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. 2006 Aug;17(8):3613–3624. doi: 10.1091/mbc.E06-04-0291

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© 2006 by The American Society for Cell Biology

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Figure 3. — Location of REX2 in acetone fixed IRBCs. (A) Specificity of the anti-REX2 serum: anti-REX2 serum reacted only with 3D7 (left) but not D10 parasites (right). Merges with nuclear stain (Hoechst) are shown. (B) IFAs with 3D7 parasites expressing a myc-tagged ETRAMP4 (Spielmann et al., 2006) showed that REX2 (green; Cy2) has a dotty appearance outside of the PVM (ETRAMP4myc in the PVM detected by a rabbit anti-myc antibody; red, Texas Red). No REX2 signal was detectable in D10 parasites expressing ETRAMP4myc (D). (C) REX2 (green; Cy2) colocalized with the Maurer’s cleft protein REX1 (Hawthorne et al., 2004) (red; Texas Red) in 3D7 parasites. Bars, 5 μm.