TABLE 2.

Sequences and melting temperatures (T_m) of primers used in RT-PCR

Primer	Sequence	Tm (°C)
RTA3-F	5′-CGAAAGCAAACCAAGTCCAT-3′	54.9
RTA3-R	5′-TACCAATCATTGCTGCATCC-3′	54.3
IPF3121-F	5′-TTGCCACCACCTAATCATCA-3′	55.3
IPF3121-R	5′-CAACAGGTTCCGTTTGTGTG-3′	55.6
DDR48-F	5′-TTTCGGTTTCGGTAAAGACG-3′	54.1
DDR48-R	5′-TGAGTCGGTCTTGGAGGAAC-3′	57.2
EBP1-F	5′-GCTTATGTCCCAACCACCAG-3′	56.7
EBP1-R	5′-CTGATGGCGCAATAAGAGGT-3′	56.1
CDR1-F	5′-GGCAATTAGTCAAGACTCTTCTTCAG-3′	57.2
CDR2-F	5′-CAGCTAGACGAAAAGCCATGG-3′	57.1
CDR-Ra	5′-CACCTGGTCTCATAATGGCATC-3′	56.7
IFD1-F	5′-CGACAATGGCTTCCGTACTT-3′	56.2
IFD4-F	5′-GATTGGGATCCAGTTGGAGA-3′	55.0
IFD5-F	5′-GGGAAGATTTTGATCCTGTTGATAG-3′	55.2
LPG20-Rb	5′-CACCAGCAATTGGAATCACA-3′	54.7
IFD7-F	5′-AGATGGTTTGGGTGAAGTCG-3′	56.1
IFD7-R	5′-CACCGGCAATTGGGACAACTCC-3′	62.1
GRP2-F	5′-ACATACCGCATCTCCATTCC-3′	55.6
GRP2-R	5′-TGGAACCAACATAACCAGCA-3′	55.7
DPP1-F	5′-TCAACCATTTGCACGACAAT-3′	54.6
DPP1-R	5′-TCAGGAGTTCCCTTTTGTGG-3′	55.7
CRD2-F	5′-GCTTGTTCTGCTGCTCAATG-3′	55.8
CRD2-R	5′-TTTCACCATCTCTAGTACCATCACA-3′	57.1
INO1-F	5′-GATTTCAAGGCCAAAAACGA-3′	53.0
INO1-R	5′-TTTGATACCAGCATCGACCA-3′	55.2
IPF1222-F	5′-CTCCAAAGGCAGTCCATCAT-3′	55.9
IPF1222-R	5′-TTGTGTTAGCATTGCCGTGT-3′	56.8
OPI3-F	5′-GGCACAAAATTTCCCACAAT-3′	53.4
OPI3-R	5′-GCAAACAATGCAATGGCTAA-3′	53.7
MDR1-F	5′-TTTTTGGGTGGATTCTTTGC-3′	53.0
MDR1-R	5′-CGGTGATGGCTCTCAATCTT-3′	55.8
18S rRNA-F	5′-GCC AGC GAG TAT AAG CCT TG-3′	56.7
18S rRNA-R	5′-AGG CCT CAC TAA GCC ATT CA-3′	57.3

^aThe CDR-R primer is used with both the CDR1-F and CDR2-F primers.

^bThe LPG20-R primer is used with IFD1-F, IFD4-F, and IFD5-F primers.