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HSF1 S303/7D is preferentially sumoylated in vitro. In vitro-translated, 35S-labeled HSF1 mutants and androgen receptor (AR) were incubated with purified GST-SUMOGG-1 (SUMO-1) or GST-SAE1/GST-SAE2 (SAE1/SAE2) in the presence or absence of GST-Ubc9 as indicated. Reaction products were resolved by SDS-PAGE, and the signal was visualized by fluorography. Sumoylation is detected as the appearance of slower-migrating bands. WT, wild type.
