FIG. 3.

Genetic interaction of POL2 and TRF4. POL2 and DPB2 suppress the cold sensitivity in a trf4Δ mutant. Plasmids PDLT4, pSEY18-II, pSEY18-DPB2, and pSEY18 (GAL1 and GAL10 expression vectors with inserts of TRF4, POL2, DPB2, or empty [no insert], respectively) were transformed into strains 6265 (BY4741-trf4Δ) and the wild type (BY4741) and selected for growth on synthetic medium with dextrose-Ura. The resultant colonies were purified, grown to log phase in liquid SRaff-URA, and serially diluted onto plates containing 2% galactose. Duplicate plates were incubated at 16°C for a week.