FIG. 7.

Sister chromatid separation in a pol2 mutant begins earlier in the cell cycle than in the wild type. Wild-type cells (AFS479) and pol2-12 cells (JCY122) were grown to log phase at 30°C, induced for GFP, and then arrested with α-factor for 3 h. Cells were then released in YPD at 30°C (A and B) or 34°C (C). Samples were taken every 15 min and analyzed for bud emergence by differential interference contrast (represented as budding index), timing of sister chromatid separation by GFP assay, spindle elongation by indirect immunofluorescence using an antitubulin antibody, DNA content by flow cytometry, and nuclear morphology by DAPI staining.