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. 2003 Apr;23(8):2733–2748. doi: 10.1128/MCB.23.8.2733-2748.2003

TABLE 1.

Yeast strains

Strain Genotype Source
AFS479 MATaura3-1 ade2-1 can1-100 trp1-1 leu2-3,112::lacO-256 (pAFS59)-LEU2+ his3-11,15::HIS3 [-GFP-Lac1(pAFS144)-HIS3+] A. Straight
L40 MATahis3Δ200 trp1-901 leu2-3,112 ade2 lys2-801am URA3::(lexAop)8-lacZ LYS2::(lexAop)4-HIS3 Hollenberg, 1995
BY4741 MATahis3-D1 leu2-D0 met15D0 ura3D0 Research Genetics
1145 BY4741 trf5Δ Research Genetics
6265 BY4741 trf4Δ
BY-pol2-12 MATahis3-D1 leu2-D0 met15D0 ura3D0 pol2-12 This study
BY-pol2-11 MATahis3-D1 leu2-D0 met15D0 ura3D0 pol2-11 This study
BY-trf4Δpol2-11 MATa6265 trf4Δpol2-11 This study
BY-trf5Δpol2-12 MATa1145 trf5Δpol2-12 This study
BY-trf5Δpol2-11 MATa1145 trf5Δpol2-11 This study
985-7C MATamcd1-1 trp1 ura3 bar1 gal1 D. Koshland, 1997
955-9C MATα mcd1-1 trp1 ura3 bar1 gal1 D. Koshland, 1997
YPH102 MATα ura3-52 lys2-801 ade2-101 his3-200 leu2-1 P. Heiter
Y414 YPH102 pol2-12 This study
3aAS273 YPH102 smc1-2 V. Guacci, 1997
3aAS273a MATaura3-52 lys2-801 ade2-101 his3-200 leu2-1 smc1-2 This study
CY1243 MATatrf4ts896-HIS3 trf5::LEU2 (his3-11,15) (leu2-3,112) trp1-1 ade2-1 ura3-1 Castano and Christman, 1996
TC102-2-12 MATaleu2 ura3-52 can1 pol2-12 Budd and Campbell, 1993
SS111 MATatrp1-289 ura3-1,2 ade2-101 gal2 can1 his3 Budd and Campbell, 1993
SS111-2-12 SS111 pol2-12 Budd and Campbell, 1993
SOG3 MATatrp1-289 ura3-1,2 ade2-101 gal2 can1 lacO-256 (pAFS52)-TRP1+ his3-11,15::HIS3 [-GFP-Lac1(pAFS144)-HIS3+] This study
12OG2 SOG3 pol2-12 This study
HKYDNA2 MATadna2-2::LEU2 ura3-1 ade2-1 can1-100 trp1-1 leu2-3,112 H. Klien
JCYDNA2 HKYDNA2 lacO-256(pAFS52)-TRP1+ his3-11,15::HIS3 [-GFP-Lac1(pAFS144)-HIS3+] This study
ts370 MATaade1 ade2 ura1 his7 tyr1 gal1 cdc2-1 Budd and Campbell, 1993
JCYT59 SOG3 trf5Δ This study
JCY122 AFS479 pol2-12 This study
JCY123 JCY122 × 3aAS273a This study
JCY124 JCY122 × 955-9c This study
JCY125 JCY122 × CY1243 This study
JCYPD18 ts370 lacO-256(pAFS52)-TRP1+ his3-11,15::HIS3[-GFP-Lac1(pAFS144)-HIS3+] This study