TABLE 4.
Two-hybrid assaysa
| Protein | β-Galactosidase activity (U/mg)
|
|||||||
|---|---|---|---|---|---|---|---|---|
| AD | POL C-terminal-AD | TRF4-AD | TRF5-AD | DPB2-AD | DPB11-AD | SMC1-AD | ECO1-AD | |
| BD | 1.2 | − | − | − | − | − | − | − |
| POL2-C-terminal-BD | − | + | 22.3 | 5.8 | + | + | + | + |
| TRF4-BD | − | 1.1 | − | + | 0.6 | 2.3 | ND | ND |
| TRF5-BD | − | 33.4 | + | − | 16.0 | 36.0 | ND | ND |
| DPB2 | − | + | 0.8 | 9.7 | + | + | ND | ND |
a
Numerical values are based on quantitative assays of β-galactosidase activity (Stratagene; see Materials and Methods) and were measured in the same experiment and therefore are comparative. + indicates positive β-galactosidase activity measured using a filter lift assay only (Clontech). Activation of β-galactosidase activity in these samples was positive for blue color, indicating interaction of the genes tested. ND, not done; AD, activation domain (Gal4); BD, binding domain (LexA). Quantitative assays were not done with these clones. − indicates no β-galactosidase activity, represented by no color formation in a filter lift assay. Therefore, no interaction was observed.