TABLE 5.
Two-hybrid mapping of Trf5 interaction within Pol2
| Genotype or region | % β-Galactosidase activity for BD-TRF5a |
|---|---|
| AD | 0 |
| Pol2 C terminus | 100 |
| pol2-A | 30 |
| pol2-B | 10 |
| pol2-E | 0 |
| pol2-F | 45 |
| pol2-H | 58 |
| pol2-I | 69 |
| pol2-11 | 35 |
| pol2-13 | 28 |
a
One hundred percent activity is determined by the interaction of the activation domain (AD) fused to the Pol2 C terminus and the binding domain (BD) fused to Trf5 minus the interaction of the empty vector pAct2-AD and BD-Trf5. All fusion genes except that for BD-Trf5 were cloned into the activation domain vector pAct2. The values are an average of two different determinations and were further confirmed by colony filter-lift color assay.