FIG. 1.
Enhancement of unmethylated CRE-dependent gene expression by MBD2a. (A) Repression of methylated Som-Luc activity by MBD2a and MBD2b. HEK-293 cells were transfected with Som-Luc reporter either after methylation with SssI methylase or after mock methylation. The change in repression on both reporter constructs was calculated relative to the luciferase activity after transfection of the empty vector and unmethylated reporter. (B) Enhancement of unmethylated Som-Luc activity by MBD2a. The luciferase activity of cells transfected with empty vector alone and PKAmut was set as 1. (C) Selective interaction of MBD2a with RHA in yeast. Yeast two-hybrid experiments were performed with MBD2a as bait (GAL4 fusion) and CREB, CBP, RHA1 to RHA4, and hTAFII130 as prey (fusion to the activation domain).