FIG. 2.

Interaction of MBD2a, but not MBD2b, with RHA. (A) Schematic representation of MBD2a and MBD2b. GR₁₁, glycine-arginine repeats; MBD, methyl-CpG binding domain. (B) No association of MBD2b with RHA in yeast. Yeast two-hybrid experiments were performed with MBD2a and MBD2b as bait and RHA1 as prey. (C) Binding of GST-MBD2a to RHA in vitro. The GST fusion proteins were expressed in E. coli, purified, and incubated with in vitro-translated, ³⁵S-labeled RHA. (D) Interaction of MBD2a with RHA in vivo. A Western blot of HA shows the immunoprecipitation of anti-HA antibody from HEK-293-transfected nuclear extracts. The asterisk indicates immunoprecipitated IgG heavy chain.