FIG. 5.

Identification of the transactivation domain in the C terminus of MBD2. (A) Schematic representation of deletion mutants of MBD2. (B and C) Transactivation assays were performed with yeast (B) and mammalian (C) cells transformed with MBD2 deletion mutants. The β-Gal or luciferase activity of each mutant was compared to that of the empty vector alone, which was set as 1. Each value of relative β-Gal or luciferase activity represents the mean ± standard error (n = 3). (D) Effects of each domain within MBD2 on CRE-dependent gene expression. HEK-293 cells were cotransfected with Som-Luc and the plasmids expressing various MBD2 mutants. The luciferase activity of cells cotransfected with empty vector alone and unmethylated Som-Luc was set as 1. Each value of relative luciferase activity represents the mean ± standard error (n = 3).