FIG. 1.

NPAT is transcriptionally up-regulated in NIH 3T3 cells following growth stimulation. (A) Cell cycle distribution profiles for NIH 3T3 cells during quiescence and serum stimulation. NIH 3T3 cells were serum starved and restimulated to enter the cell cycle as described in Materials and Methods. At the indicated times after serum addition, the cell cycle distribution of the cells was analyzed by FACS. (B) Analysis of NPAT mRNA expression in NIH 3T3 cells treated as described for panel A. NPAT mRNA was analyzed by Northern blotting as described in Materials and Methods. The analysis of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the same samples as a control is also shown.